 | From Dr. Chris Lytle's lab: Confocal immunofluorescence image of mouse distal colon showing abundant K-Cl cotransporter-1 (green label) in smooth muscle cells of the muscularis and blood vessels.
| From Dr. Chris Lytle's lab: A low-power cross-sectional view of the mouse colon showing the inner “glandular” layer (called the mucosa) and the outer muscular layers.
|  |  | This image from Dr. Chris Lytle's lab shows that the colonic epithelium is also in a state of perpetual renewal. As the cells migrate out of the crypts, they abruptly lose machinery for chloride secretion (such as NKCC1, labeled red), and acquire machinery for sodium absorption (including Na/H exchanger-3, labeled green).
| This image, which resulted from a collaboration between the labs of Dr. Chris Lytle and Dr. Katie DeFea, shows the epithelial layer of a mouse small intestine (jenunum). Newly formed cells begin life in crypts uniquely specialized for chloride secretion, with an abundance of Na-K-2Cl cotransporter-1 (NKCC1, labeled red). As the migrating cells begin to emerge from the crypt, they undergo a remarkable transformation, abruptly losing secretory transport machinery and acquiring numerous absorptive transporters including NHE3 and H,K-ATPase. The intestinal epithelial cells also contain high levels of protease-activated receptor-2 (PAR-2, labeled green), particularly along their apical margins. Nuclei are labeled blue.
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| This photo, from the lab of Dr. Iryna Ethell, is a confocal image of GFP-labeled spiny hippocampal neuron in 21-day-old culture. For more information, contact Dr. Ethell at
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. | | This image is from a collaboration between the labs of Dr. Iryna Ethell and Dr. Monica Carson. Confocal images of mixed cultures of microglia (green) and 14-day-old hippocampal neurons (red). Immunodetection of microglia with CD11b (green), neurons with MAP2 (red) and cell nuclei with DAPI (blue). |  |  | Another stunning image from Dr. Iryna Ethell's lab. Confocal image of 14-day-old hippocampal neurons. Detection of dendrites with MAP2 (red) and nuclei with DAPI (blue).
| The 2005 Walker lab crew: From bottom left to right, Eric Ueda, Ariel DeGuzman, Dunyong Tan, Changhui Deng, Ameae Walker, Mary Lorenson, Wei Wu, YenHao Chen, Valencia Williams, Lisa Ma and Mark Huang.
|  |  | Activated microglia surrounding amyloid plaques (from the lab of Dr. Monica Carson) | | Using lentiviral vectors to transduce microglia and other cells.
Here, transduced cells are green due to the expression of lentivirus derived green-fluoresecent protein (from the lab of Dr. Monica Carson) |  |  | Dr. Carson’s lab is monitoring gene expression changes induced in the hippocampus following kainic acid induced epilepsy using in situ hybridization analysis. These experiments are part of an ongoing collaboration with Dr. Naegle’s Lab at Wesleyan University. |
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